Acquisition, and analysis instruction manual bd cba flex set setup manual for bd. Is there any good alternative to bd biosciences cytometric bead. Software bd facsarray software controls instrument setup and sample acquisition. The data files are analyzed using bd cba calibration and analysis software. Not for use in diagnostic or therapeutic procedures. The software also has broad compatibility as it is offered in both pc and maccompatible formats, requiring only microsoft excel to run. The intuitive analysis software generates a numerical readout in. Application flow cytometry routinely tested regulatory status ruo. L of wash buffer and vortex the tube gently to mix the contents. Unless otherwise noted, bd, the bd logo and all other trademarks are property of becton, dickinson and company.
The intuitive analysis software generates a numerical readout in relative. Bd cba solutions are designed for multiplexed analysis, to provide more data using a single sample. It also supports batch analysis with customizable automated gating tools. And for analysis, has anyone been able to analyze data using flowjo vs the fcap array software by bd. Analyzing data from bd cba kits using fcap array software ii. Bd cba software provides quantitative analysis for up to eight analytes beads per sample file.
L of cytometer setup beads vial d to a 12 x 75mm sample acquisition tube. Bd cba solutions are designed for multiplexed analysis, to provide more data using a single. Each concentration level must have the same number of replicates. With bd cba, up to 30 proteins can be analyzed using just 25 to 50. Cba is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Looking for online definition of cba or what cba stands for. Multiplexing is especially useful when only a small amount of sample is available, maximizing the number of proteins that can be analyzed. If so, would greatly appreciate any protocols out there. Bd cytometric bead array cba is a flow cytometry application that allows users to.
This site supported in part by national science foundation grant ast1615456 to j. Bd biosciences offers both pc and macintosh software for flow analysers and cell sorters enabling data acquisition, data analysis and integrated applicationspecific modules. Brand bd cytometric bead array cba contents installation dvd, hardware key, users guide. The bd cba software enables linear regression analysis of data files and extrapolation of sample values by comparison against a known standard curve. Guide to analyzing data from bd cytometric bead array cba.
Data handling options include data export into other bd analysis, thirdparty analysis, or database software applications. There can be up to 20 calibration concentration levels for the standards, each with up to five replicates. Bd facsarray software is compatible with fcap array. Guide to using bd facsdiva software with bd cytometric. If anyone has a protocol to analyze the bd cba data without using the software i. Bd cytometric bead array brochure fisher scientific.